|Lay, Jr, Donald - Don|
Submitted to: Journal of Animal Science
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2006
Publication Date: 7/9/2006
Citation: Moulton, K., Williams, E., Ryan, P., Moore, D., Kim, S., Lay Jr, D.C., Willard, S. 2006. Detection of photonic emissions with varying concentrations of salmonella typhimurium – lux through porcine intestinal tissue: a comparison of two photonic imaging systems. Journal of Animal Science. 84(1):349.
Technical Abstract: The objective of this research was to correlate photonic emissions with concentration of Salmonella typhimurium (S. typh-lux; transformed with plasmid pAK1-lux) using the Berthold/NightOwl (BNO) and Stanford Photonic (SP) imaging systems in porcine intestinal tissues. Porcine small and large intestines were obtained after harvest and cleaned of digestive materials. Two intestinal segments were imaged per session and 18 images were collected in both the SP and BNO imaging systems (1 to 60 s and 3 to 300 s, respectively). Varying concentrations of bacteria were used in small and large intestine studies, ranging from 5.7 x 105 to 8.0 x 107 CFU/ml. For each experiment, one segment was empty for a background count and one segment was injected with S. typh-lux broth; the difference of which was used in analysis. Throughout imaging (up to 6 replicates) S. typh-lux broth was serially diluted, plated, and 24 h later counted to determine concentrations of bacteria imaged. Images from the SP and BNO systems were analyzed using Image J (NIH) and WinLight software, respectively. Data analysis included correlations between imaging systems, photonic emissions and bacterial concentrations. From small intestines, photonic emissions were positively correlated with bacterial concentration in SP (R=0.80) and BNO (R=0.60; P<0.05) systems. The correlation between imaging systems in small intestines relative to photonic emissions was 0.73 (P<0.05). From large intestines, photonic emissions were positively correlated with bacterial concentration in SP (R=0.52) and BNO (R=0.60; P<0.05) systems. The correlation between imaging systems in large intestines relative to photonic emissions was 0.65 (P<0.05). These data indicate that concentrations of S.typh-lux are highly correlated with photonic emissions, and may be detected through porcine intestinal tissues using SP or BNO imaging systems. [USDA-NRI grant # 2003-35201-13841; USDA-ARS funded Biophotonic Initiative # 58-6402-3-0120]