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ARS Home » Southeast Area » Stoneville, Mississippi » Biological Control of Pests Research » Research » Publications at this Location » Publication #192327


item Allen, Margaret - Meg

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 2/21/2006
Publication Date: 12/15/2006
Citation: Allen, M. L. 2006. Expressed Sequence Tags from a Lygus lineolaris (Palisot de Beauvois) (Heteroptera: Miridae) Male Nymph EST Library. Genbank.

Interpretive Summary: Plant bugs belong to a general group of crop pests known as “sucking pests”. Genetically modified crops are presently ineffective against these pests, so plant bugs and other sucking pests must be managed with insecticide treatments. New, gene-based pest control methods require some understanding of the insect genes associated with feeding, digestion, and nutrition. Before now, few genes have been identified from this type of insect. This limits both development and testing of new gene-based pesticides. To begin addressing this problem, gene sequences were collected from an actively feeding stage of the tarnished plant bug, Lygus lineolaris, and have been filed in public data repositories. This information will provide scientist with genetic tools to study populations of plant bugs and target feeding genes for disruption.

Technical Abstract: Few gene sequences have been isolated from the tarnished plant bug, Lygus lineolaris, an important pest of many crops including cotton. This lack of data limits the study of important functions such as feeding, digestion, development, and reproduction. Gene expression studies can provide specific information to facilitate these functional studies, and provide genetic markers for identification and monitoring studies. Over 250 sequences were prepared from an EST library from male nymph Lygus lineolaris polyA RNA. This is a preliminary or pilot library targeting a feeding stage, designed to sample insect genes prior to adult gene expression associated with reproduction. The genetic products deposited in the National Center for Biotechnology Information (NCBI) GenBank support efforts to manage this increasingly important crop pest.