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Title: BIOCONVERSION OF N-6 AND N-3 PUFAS BY BACILLUS MEGATERIUM ALA2

Author
item HOU, CHING

Submitted to: International Society for the Study of Fatty Acids and Lipids
Publication Type: Abstract Only
Publication Acceptance Date: 7/28/2006
Publication Date: 7/28/2006
Citation: Hou, C.T. 2006. Bioconversion of N-6 and N-3 pufas by Bacillus megaterium ALA2 [abstract]. International Society for the Study of Fatty Acids and Lipids. S-29. p. 43.

Interpretive Summary:

Technical Abstract: We have been working on producing value-added products from vegetable oils by biocatalysis including healthy oils and new oxygenated fatty acids. Oxygenated fatty acids can be used as specialty chemicals and biomedicals. Bacillus megaterium ALA2 (NRRL B-21660) converted n-6 and n-3 PUFAs to value-added oxygenated products. Linoleic acid was converted to: trihydroxy-9(S)-octadecenoic acid (THOA), diepoxy-bicyclic unsaturated fatty acid (DEOA), and tetrahydrofuranyl unsaturated fatty acid (TFOA) with 12,13,17-THOA as the main product. The linoleic acid bioconversion pathway by strain ALA2 was established. Strain ALA2 also converted EPA, DHA, arachironic acid and '-linolenic acid to new oxygenated products including DEOAs, TFOAs, and THOAs. Recently, we found that strain ALA2 possesses a monooxygenase system oxidizing palmitic acid to omega-1, omega-2, and omega-3 hydroxy palmitic acids similar to those reported in the well-studied catalytically self-sufficient P450 monooxygenase of Bacillus megaterium ATCC14581 and B. subtilis. We also found that strain ALA2 produced 9,10-epoxy-12-(Z)-octadecenoic acid from linoleic acid. These findings enable us to compare and utilize known Bacillus gene data with our strain ALA2 for metabolic pathway engineering to produce products of interest.