Submitted to: Journal of Cereal Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/16/2005
Publication Date: 4/30/2005
Citation: Chen, F., He, Z., Xia, X., Lillemo, M., Morris, C.F. 2005. a new puroindoline b mutation present in chinese winter wheat cultivar jingdong 11. J. Cereal Sci. 42:267-269. Interpretive Summary: A new puroindoline gene mutation has been discovered in Chinese winter wheat variety Jingdong 11. This allele (named PINb-D1q) is likely the cause of hard grain texture in Jingdong 11. Kernel hardness is an important trait that in part determines the use and commercialization of bread wheat. This discovery will also play an important role in the genetic improvement of wheat.
Technical Abstract: Kernel hardness is one of the most important characteristics in determining utilization and marketing of bread wheat. Genes coding for puroindoline a and b (PINA and PINB) were located at the Ha locus and designated as Pina-D1 and Pinb-D1, respectively. The coding sequence of the Pinb gene in a Chinese winter wheat cultivar Jingdong 11 (Triticum aestivum L.) was amplified with polymerase chain reaction (PCR), and the obtained 447-bp fragment sequenced from two strands, and compared with the eight known Pinb alleles. The results showed that Jingdong 11 possessed a new Pinb allele not reported previously, and was designated as PINb-D1q. It is characterized by a single base T to G substitution, which results in a tryptophan to leucine substitution (TGG to TTG) at position 44 and is most likely the cause of hard grain texture in Jingdong 11. The characterization of Pinb-D1 alleles would be helpful in manipulating grain hardness of bread wheat in breeding programs.