|Can baser, K. husnu|
Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer reviewed journal
Publication Acceptance Date: 3/5/2006
Publication Date: 3/23/2006
Citation: Demirci, B., Can Baser, K., Tabanca, N., Wedge, D.E. 2006. Characterization of volatile constituents of haplopappus greenei and studies on the antifungal activity against phytopathogens. Journal of Agricultural and Food Chemistry. 54:3146-3150. Interpretive Summary: Essential oil of Haplopappus greenei was obtained from above ground parts and evaluated for antimalarial, antimicrobial, and antifungal activities. One hundred and four compounds were identified and carvacrol, B-pinene, trans-pinocarveol, and caryophyllene oxide were found to be the most abundant compounds. Antifungal activity was observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloesporioides using direct bioautography assays. Carvacrol, a major component of the essential oil, demonstrated strong antifungal activity.
Technical Abstract: Essential oil of Haplopappus greenei A. Gray was obtained by hydrodistillation of aerial parts, which were subsequently analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Major components were identified as carvacrol (8.7%), B-pinene (7.6%), trans-pinocarveol (6.2%), and caryophyllene oxide (5.8%), respectively. In total, 104 components representing 84.9% of the investigated essential oil were characterized and further evaluated for antimalarial, antimicrobial, and antifungal activities. However, antifungal activity was observed against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae and C. gloesporioides using the direct overlay bioautography.