Submitted to: Rice Technical Working Group Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 1/1/2006
Publication Date: 2/5/2006
Citation: Jia, Y., Singh, P., Jia, M.H., Wang, G., Wamishe, Y., Zhu, L., Zhou, E. 2006. Development of molecular strategies to control rice sheath blight disease [abstract]. In: Rice Technical Working Group Meeting Proceedings, February 28 - March 1, 2006, Houston, Texas. 2006. CDROM.
Technical Abstract: Little is known about mechanisms of molecular interaction of host with the necrotrophic pathogen Rhizoctonia solani. After a detailed analysis of the pathogen population in the major rice producing state, Arkansas, the most virulent field isolate was identified among 124 characterized isolates. The most virulent field isolate was used to inoculate a cultivar, Jasmine 85, which contains minor resistance genes to the pathogen. Messenger RNAs 16 hours after inoculation were extracted to detect key components for the interaction. Experiments were repeated with three different molecular techniques: 1) Subtractive suppression hybridization (SSH) library, 2) DNA microarray, and 3) Robust Long serial analysis of gene expression (RL-SAGE). Thus far, 200 expressed genes were analyzed from a SSH library, a profile of 22,000 rice genes from DNA microarray, and 6720 SAGE clones of the treated and the control each containing 40-45 tags. Our current analysis suggests that a sophisticated interaction between pathogen secreted proteins and cell wall associated host proteins may occur during early infection and these interacting genes may be key targets for the control of rice sheath blight disease.