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United States Department of Agriculture

Agricultural Research Service

Title: FUNCTIONAL GENOMIC ANALYSIS OF THE AUXIN/INDOLE-3-ACETIC ACID GENE FAMILY MEMBERS IN ARABIDOPSIS THALIANA)

Author
item Overvoorde, Paul
item Okushima, Yoko
item Alonso, Jose
item Chan, April
item Chang, Charlie
item Ecker, Joseph
item Hughes, Beth
item Liu, Amy
item Onodera, Courtney
item Quach, Hong
item Smith, Alison
item Yu, Guixia
item Theologis, Athanasios

Submitted to: The Plant Cell
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/3/2005
Publication Date: 11/11/2005
Citation: Overvoorde, P.J., Okushima, Y., Alonso, J.M., Chan, A., Chang, C., Ecker, J.R., Hughes, B., Liu, A., Onodera, C., Quach, H., Smith, A., Yu, G., Theologis, A. 2005. Functional Genomic Analysis of the AUXIN/INDOLE-3-ACETICc ACID Gene Family Members in Arabidopsis thaliana. The Plant Cell. 17:3282-3300.

Interpretive Summary: Auxin regulates various aspects of plant growth and development. The AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) genes regulate auxin-mediated gene expression by interacting with members of the AUXIN RESPONSE FACTOR protein family. Aux/IAA function is poorly understood; herein, we report the identification and characterization of insertion mutants in 12 of the 29 Aux/IAA family members. The data suggest extensive functional redundancy among Aux/IAA gene family members and that enhanced stability of the AXR3/IAA17 protein severely alters the molecular phenotype, resulting in developmental defects.

Technical Abstract: Auxin regulates various aspects of plant growth and development. The AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) genes encode short-lived transcriptional repressors that are targeted by the TRANSPORT INHIBITOR RESPONSE1/AUXIN RECEPTOR F-BOX proteins. The Aux/IAA proteins regulate auxin-mediated gene expression by interacting with members of the AUXIN RESPONSE FACTOR protein family. Aux/IAA function is poorly understood; herein, we report the identification and characterization of insertion mutants in 12 of the 29 Aux/IAA family members. The mutants show no visible developmental defects compared with the wild type. Double or triple mutants of closely related Aux/IAA genes, such as iaa8-1 iaa9-1 or iaa5-1 iaa6-1 iaa19-1, also exhibit wild-type phenotypes. Global gene expression analysis reveals that the molecular phenotypes of auxin-treated and untreated light-grown seedlings are unaffected in the iaa17-6 and iaa5-1 iaa6-1 iaa19-1 mutants. By contrast, similar analysis with the gain-of-function axr3-1/iaa17-1 mutant seedlings reveals dramatic changes in basal and auxin-induced gene expression compared with the wild type. Expression of several type-A ARABIDOPSIS RESPONSE REGULATOR genes and a number of genes involved in cell wall biosynthesis and degradation is repressed in axr3-1/iaa17-1. The data suggest extensive functional redundancy among Aux/IAA gene family members and that enhanced stability of the AXR3/IAA17 protein severely alters the molecular phenotype, resulting in developmental defects.

Last Modified: 05/24/2017
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