Submitted to: Review Article
Publication Type: Other
Publication Acceptance Date: 2/1/2006
Publication Date: 6/11/2006
Citation: Jia, Y., Singh, P., Wamishe, Y., Jia, M.H., Gowda, M., Channaryappa, V.R., Zhou, E., Flowers, C., Lin, M.J., Wang, G.L., Rutger, J.N. 2006. Characterization of sheath blight pathogen Rhizoctonia solani and its molecular interaction with Oryza sativa. Bulletin for Biochemistry and Biotecnology of SAAS. Available: http//orgs.tntech.edu/saas/.
Technical Abstract: Abstract: Little is known about mechanisms of molecular interaction of hosts with the necrotrophic pathogen Rhizoctonia solani. Detailed analysis of the pathogen population in Arkansas, the major rice producing state in the USA, led to the identification of the most virulent field isolate out of a total of 124 isolates. The most virulent field isolate was used to inoculate a cultivar, Jasmine 85, that contains minor resistance genes. Messenger RNAs 16 hours after inoculation were extracted to detect key genetic components for the interaction. Experiments were repeated with three different molecular techniques: 1) DNA microarray, 2) Robust Long serial analysis of gene expression (RL-SAGE), and 3) Subtractive suppression hybridization (SSH) library. Thus far, 200 expressed genes have been characterized from an SSH library, a profile of 22,000 rice genes from DNA microarrays, and 6720 SAGE clones of the treated and the control, each containing 40-45 tags, were analyzed. Our current analysis suggests that a sophisticated interaction between pathogen secreted proteins and cell wall associated host proteins may occur during early infection and these interacting genes may be key targets for the control of rice sheath blight disease.