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Submitted to: United States Animal Health Association Proceedings
Publication Type: Proceedings Publication Acceptance Date: 11/8/2005 Publication Date: 1/20/2006 Citation: Wilson, W.C. 2006. Update on btv persistence in culicoides sonorensis. United States Animal Health Association Proceedings. Interpretive Summary: Molecular biology of bluetongue and related viruses has provided the foundation for the development early warning technologies for indigenous and exotic disease outbreaks. The phylogenetic analysis of two-conserved target genes, one that is highly expressed in infected mammalian cells the other highly expressed in infected insect cells, from BTV prototype strains indicated that a complex primer design will be necessary for a comprehensive gene amplification diagnostic test. Status of the application of real-time RT/PCR and other existing and developing technologies for early warning of an exotic BTV outbreak was discussed. Technical Abstract: Molecular biology of bluetongue and related viruses has provided the foundation for the development early warning technologies for indigenous and exotic disease outbreaks. The phylogenetic analysis of two-conserved target genes, one that is highly expressed in infected mammalian cells the other highly expressed in infected insect cells, from BTV prototype strains indicated that a complex primer design will be necessary for a comprehensive gene amplification diagnostic test. Status of the application of real-time RT/PCR and other existing and developing technologies for early warning of an exotic BTV outbreak was discussed. |
