|Rinehart, Timothy - Tim|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/27/2006
Publication Date: 7/30/2006
Citation: Devries, R.E., Trigiano, R.N., Windham, M.T., Windham, A., Rinehart, T.A. 2006. Genetic diversity of tennessee sclerotinia homoeocarpa isolates delineated by vegetative compatibility and aflp analysis. American Phytopathological Society Abstracts vol 41, pg 1077 New Orleans, July 27-30.
Technical Abstract: Sclerotinia homoeocarpa lesions cause serious problems by tainting the uniformity and aesthetic value of putting greens. Genetic variation between fungicide resistant dollar spot isolates collected from golf courses across Tennessee was examined by vegetative compatibility pairings, sequencing of conserved genes, and AFLP analysis. Isolates were paired against six tester strains and each other on potato dextrose agar. Most isolates were incompatible with each other and only a few were placed into vegetative compatibility groups. Sequencing of conserved genes showed 100% homology between isolates. AFLPs were detected by capillary gel electrophoresis and isolates were determined to be 80-90% similar. Although incompatibility may indicate high genetic diversity, little variability was demonstrated using molecular methods. Increased sampling of the genome will be performed to uncover sources of variation.