Submitted to: Biotechniques
Publication Type: Peer reviewed journal
Publication Acceptance Date: 6/29/2005
Publication Date: 9/1/2005
Citation: Shigake, T., Kole, M., Ward, J.M., Sze, H., Hirschi, K. 2005. Cre-loxp recombination vectors for the expression of Riken Arabidopsis full-length cDNAs in plants. Biotechniques. 39(3):301-303. Interpretive Summary: The methodology to move DNA from one place to another is often arduous. In this report, we have replaced the previously cumbersome techniques of transferring DNA into plants. We describe new tools that aid and simplify the cloning process. This should be of a general use to all plant scientists involved in genetic engineering.
Technical Abstract: An essential and often arduous task in plant functional genomic studies is the expression of full-length cDNAs in plants. Historically, this step has been confounded by technical hurdles, including obtaining full-length cDNAs and cloning these cDNAs into frequently cumbersome plant expression vectors. The Arabidopsis full-length cDNAs available from Riken (RAFL clones; Riken, Saitama, Japan) are now an invaluable resource for functional studies. To date, these inserts have been engineered into binary vectors by traditional restriction digestion and ligation. To overcome this limitation, we have developed a system that can streamline the expression of RAFL cDNAs in plants.