Submitted to: Journal of Clinical Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/2/2005
Publication Date: 1/5/2006
Citation: Lui, D., Lawrence, M.I., Gorski, L.A., Mandrell, R.E., Ainsworth, A.J., Austin, F.W. 2006 Further evidence that listeria monocytogenes serotype 4b strains belonging to lineages i and iii possess distinct molecular features. Journal of Clinical Microbiology. 72:776-783. Interpretive Summary: Listeria monocytogenes serotype 4b strains can be separated genetically into 2 distinct groups called Lineage I and Lineage III. This paper describes analysis of the two types of serotype 4b strains with PCR primers for specific genes previously found to differentiate L. monocytogenes subtypes. It was found that the Lineage I 4b strains had different PCR patterns than the Lineage III 4b strains, indicating that serotype 4b strains belonging to Lineages I and III possess distinct molecular features.
Technical Abstract: A collection of Listeria monocytogenes serotype 4b strains belonging to lineages I and III were examined by PCR and Southern blot using species-, virulence- and serotype-specific primers and probes. Whereas four serotype 4b lineage I strains reacted in PCR with serotypes 4b-, 4d- and 4c-specific ORF2110, virulence-specific lmo1134 and lmo2821 primers, all nine serotype 4b lineage III strains were negative by ORF2110 and lmo1134 primers. In addition, the nine serotype 4b lineage III strains were further divided into two separate groups through their reactions in PCR with virulence-specific lmo2821 primers. Southern blot analysis using species-specific lmo0733 and virulence-specific lmo2821 gene probes largely confirmed the PCR results. These findings indicate that L. monocytogenes serotype 4b strains belonging to lineages I and III possess distinct molecular features.