Submitted to: American Mosquito Control Association
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/9/2005
Publication Date: 9/1/2007
Citation: Xue, R.D., Ali, A., Crainich, V., Barnard, D.R. 2007. Oviposition deterrence and larvicidal activity of three formulations of piperidine repellent (ai3-37220) against field populations of stegomyia albopicta. American Mosquito Control Association, 23(3):283-287, 2007
Interpretive Summary: In North America the Asian Tiger mosquito bites animals and humans and is associated with the transmission cycle for dengue fever, eastern equine encephalitis and West Nile virus. Female mosquitoes lay their eggs in inconspicuous locations, such as tree holes, flower pots, plastic bottles, cemetery vases/urns, and discarded tires. This egg-laying behavior makes it difficult to control the mosquito because the developmental sites cannot be easily located and treated with larvicides, and because re-application of larvicide is necessary to sustain adult mosquito control. In an effort to solve this problem, scientists at the Center for Medical, Agricultural, and Veterinary Entomology, Gainesville, FL in cooperation with scientists from the University of Florida and private industry developed controlled-release formulations of a USDA mosquito repellent that prevents oviposition and survival of Asian Tiger mosquitoes in cemetery vases for more than 11 weeks. With additional formulation improvements to extend and increase biological activity at low repellent concentrations, this technology has the potential to enable season-long control of Asian Tiger mosquitoes and other container-inhabiting mosquito species with only one treatment per mosquito season.
Technical Abstract: Two proprietary controlled release (CR) formulations (CR55 and CR56) of 20% AI3-37220 (1-(3-cyclohexen-1- ylcarbonyl)-2-methylpiperidine), in an aqueous dispersion, were compared with the same repellent formulated in water (W220) and a repellent-free control for oviposition deterrent and larvicidal activity against Stegomyia albopicta. The percent Effective Reduction (% ER) in oviposition was greatest for all formulations at 0.1% concentration when tested for 24 h in the field in 1-liter plastic containers holding 500 ml of 0.001, 0.01, and 0.1% concentrations of each formulation. In a second field test, a single application of each formulation at 0.1% in the plastic containers deterred St. albopicta oviposition completely (100% ER) for 4 wk, with % ER reducing to 38 and 36%, at wk 6 for CR56 and W220, respectively, and to 46% at wk 8 for CR55. In a 10-wk field test using the plastic containers, a 0.1% concentration of each formulation resulted in 100% Larval Reduction (LR) at 4 wk post-treatment; thereafter, CR56 provided 92-100% LR for 8 wk, and CR56 and W220, 59 and 70% LR, respectively, for 6 wk. In an 11-wk evaluation in a municipal cemetery in Gainesville, Florida, one application each at 0.1% of CR55 and CR56 in granite flower-vases resulted in 100% LR of St. albopicta for 10 wk, compared with 100% LR for 8 wk resulting from 0.1% concentration of W220. With formulation improvements to enhance efficacy at low repellent concentrations, AI3-37220 may have potential for use in the control of container-inhabiting mosquitoes.