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United States Department of Agriculture

Agricultural Research Service


item Kovalchuk, I.
item Kushnarenko, S.
item Reed, Barbara

Submitted to: Modern Horticulture: Present and Future Development
Publication Type: Proceedings
Publication Acceptance Date: 9/20/2005
Publication Date: 9/17/2005
Citation: Kovalchuk, I.Y., Kushnarenko, S.V., Reed, B.M. 2005. Cryopreservation of fruit and berry cultures in kazakhstan. Modern Horticulture: Present and Future Development. p. 25-28.

Interpretive Summary: Cryopreservation of the growing tips of plants in liquid nitrogen is an effective method for long-term storage of important fruit and berry plants. Apple, currant and raspberry shoots were cryopreserved using either fast freezing or special drying techniques. Two apple cultivars had excellent recovery from the liquid nitrogen storage following the fast freezing technique. For black currants and raspberrys the drying method worked better and one third to nearly 100% of the shoot tips grew into plants after rewarming. These techniques will lead to a cryopreserved gene bank for fruit crops in Kazakhstan.

Technical Abstract: The effectiveness of methods of vitrification and encapsulation-dehydration for cryopreservation of apical meristems of apple, black currant and raspberry was compared. The vitrification method resulted in high viability of apple meristems. Shoot regrowth of cryopreserved apple meristems was 66.1% for 'Grushovka Vernenskaya' and 70.6% for 'Voshod' cultivars. Removing NAA from the recovery medium decreased callus formation and increased direct shoot growth for apple meristems. Encapsulation-dehydration was effective for cryopreservation of black currant and raspberry germplasm. Shoot tips of 31.6 to 95.7% of these cultures survived liquid nitrogen exposure and grew shoots after rewarming. Continued improvement of these techniques will lead to a cryopreserved gene bank for fruit crops in Kazakhstan.

Last Modified: 06/25/2017
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