Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/5/2005
Publication Date: 1/14/2006
Citation: Gutierrez, O.A., Saha, S., Jenkins, J.N., MCcarty Jr., J.C., Raska, D.A., Stelly, D.M. 2006. SSR-facilitated Gossypum hirsutum L. marker localization and linkage group identification using interspecific hypoaneuploid hybrids [abstract]. Plant and Animal Genome VX Conference Abstracts. CD-ROM.
Technical Abstract: Simple sequence repeat (SSR) markers were tested across two genomically incomplete sets of interspecific F1 hybrid hypoaneuploid chromosome substitution stocks to chromosomally localize markers and linkage groups, orient linkage groups, compare results between sets and to previous reports, and further define occasionally discrepant results between cytogenetic and linkage mapping. Each set was composed of quasi-isogenic monosomic (2n=51) and arm-deficient monotelodisomic (2n=52) F1 interspecific hybrids between the Upland cotton (Gossypium hirsutum L.) inbred ‘TM-1’ and one of two species, either G. barbadense (L.) doubled-haploid line ‘3-79’ or G. tomentosum (Nutt. ex Seem.). The G. tomentosum series was developed only recently and has not been used previously for cotton genome-mapping efforts. Reliable amplification was observed for 373 of 381 BNL SSR primer pairs, of which 358 (97%) and 343 (93%) PCR products were polymorphic between TM-1 and the two species, respectively. Just four (<1%) PCR products were monomorphic. Polymorphisms were detected at 416 SSR marker loci, of which 292 (70%) were identified as deficiencies among the available hypoaneuploid stocks. Results indicated chromosomal locations of 154 SSR loci that have not been localized previously. For several markers, results from the two sets were discordant. Based on previously reported SSR locations, we observed a total of 138 SSR loci unexpectedly present and 124 unexpectedly absent. All or most of the former are most likely due to previous errors, and most of the latter remain unexplained. Locations of 18 SSR loci were determined solely by tests with the G. tomentosum set. In addition, new linkage group assignments will be presented.