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ARS Home » Southeast Area » Little Rock, Arkansas » Arkansas Children's Nutrition Center » Research » Publications at this Location » Publication #186801
Title: FEEDING OF CASEIN DIETS SUPPLEMENTED WITH BLUEBERRY OR GRAPE POWDER DURING DEVELOPMENT ALTERS HEPATIC PHASE I AND II METABOLISM IN SPRAGUE DAWLEY RATS

Author
item RONIS, MARTIN - ACNC/UAMS
item BADEAUX, JAMIE - ACNC/ACH
item SEELY, KATHRYN - UAMS
item RODGERS, BRADLEY - UAMS
item WU, XIANLI - ACNC/UAMS
item Prior, Ronald
item BADGER, THOMAS - ACNC/UAMS

Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 2/6/2006
Publication Date: 3/27/2006
Citation: Ronis, M.J., Badeaux, J., Seely, K., Rodgers, B., Wu, X., Prior, R.L., Badger, T.M. 2006. Feeding of casein diets supplemented with blueberry or grape powder during development alters hepatic phase I and II metabolism in sprague dawley rats [abstract]. The FASEB Journal. 20(4):A1015.

Interpretive Summary: Dark colored fruits such as berries and grapes are rich in a group of chemicals called polyphenols and in particular in anthocyanin pigments which make the fruit blue, purple or red. These chemicals have been suggested to have beneficial health properties including anti-cancer and heart healthy effects. In the current study, we examined the effects of feeding fruit-based diets on liver enzymes involved in cancer risk. Feeding blueberries resulted in increased activities of important enzymes that are responsible for breaking down carcinogens. No consistent effects on activity of these enzymes were observed in grape-fed rats. The activities of enzymes necessary to prepare carcinogens for excretion into the urine for disposal from the body were significantly increased by both blueberry and grape feeding, but grape was the more potent in this regard. These data suggest that consumption of berries and grapes with differing anthocyanidin profiles can alter liver metabolism in a direction that should help prevent cancer.

Technical Abstract: Fruits rich in anthocyanin pigments have been suggested to have beneficial health properties including anti-cancer and cardioprotective effects. In the current study, Sprague-Dawley rats were fed casein-based AIN-93G diets from GED4 to PND50 with or without supplementation with 2.5, 5 or 10% lyophilized powdered blueberries or concord grapes, fruits with differing anthocyanin profiles. At sacrifice, liver microsomes and cytosol were prepared and assayed for activity and expression of cytochrome P450 enzymes involved in phase I metabolism of endogenous steroids and xenobiotics and the phase II enzymes GST' and NADPH quinine reductase (QR). Feeding blueberries resulted in decreased testosterone 2'-, 16'- and 6'-hydroxylase activities in male rats (p < 0.05). The latter was accompanied by reduced expression of CYP3A apoprotein expression (p < 0.05). In addition, blueberry feeding reduced expression of CYP2E1 apoprotein (p < 0.05) in both male and female rats. No consistent effects on activity or expression of these cytochrome P450s was observed in grape-fed rats. In contrast, GST' and QR activities was increased by both blueberry and grape feeding (p < 0.05) but grape was the more potent inducer. These data suggest that feeding berries with differing anthocyanidin profiles can alter hepatic phase I and II metabolism in a specific fashion.