|Bonman, John - Mike|
Submitted to: Plant Genome Conference Proceedings
Publication Type: Abstract only
Publication Acceptance Date: 1/1/2006
Publication Date: 1/1/2006
Citation: Vemulapalli, C., Bonman, J.M., Sharma, G., Soliman, K., Kantety, R. 2006. Assessment of genetic variation in avena fatua by dna marker profiling. Plant Genome Conference Proceedings. Interpretive Summary:
Technical Abstract: Oat is an important cereal with high levels of protein and essential minerals. One of the wild oats, A. fatua, is closely related to the cultivated oat, A. sativa. Both these species are allohexaploids with AACCDD genomes and are cross fertile with each other. Therefore, A. fatua germplasm can be exploited for the improvement of cultivated oat. An understanding of morphological, genetic and molecular variation is required for the use of wild germplasm in introgression programs. The objectives of this study are i) to characterize a core subset of wild oat collection within the national small grains germplasm resource using molecular markers and ii) to determine their validity and usefulness based on comparisons to agronomic, morphological and genetic descriptors. Information generated will help guide future evaluation, maintenance, and acquisition of new materials and will benefit oat breeding programs.We isolated DNA from 83 genotypes including 64 A. fatua! accessions, one A. nuda accession and 18 A. sativa reference genotypes commonly used in oat genetic studies. One hundred and twenty seven markers including oat microsatellites and PCR-RFLP markers generated from grass anchor probes were used to screen for polymorphism using both 4% Metaphor agarose and polyacrylamide gel electrophoresis. Comparative analysis including phylogenetic relationships among accessions, association of markers with agronomic traits, and genomic regions of variability in A. fatua, will be discussed.