|Tai, Yin Shan|
Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 10/30/2005
Publication Date: 1/5/2006
Citation: Tai, Y., Faris, J.D., Friesen, T.L., Xu, S.S., Meinhardt, S.W., Stack, R.W., Simons, K., Bragg, J., Edwards, M.C. 2006. Applications of functional genomics in studies of wheat domestication and disease resistance [abstracts]. Plant and Animal Genome XIV Conference. Abstract No. P830:307.
Technical Abstract: Virus-induced gene silencing (VIGS) is a powerful genetic tool for plant research to investigate genes in a high-throughput manner. The application of VIGS in wheat has been limited by the availability of a suitable vector in monocots, although it is very commonly used in dicots. We tested three marker genes to verify the application of VIGS in wheat, using a modified vector made from Barley Stripe Mosaic Virus (BSMV). Furthermore, we confirmed decreased RNA levels of each gene by reverse-transcription polymerase chain reaction. With this VIGS tool in hand, here we tried to demonstrate its application for investigating a variety of research projects. Another useful functional genomics approach is yeast-two hybrid (Y2H) analysis. Genome-wide application of Y2H analysis to study protein-protein interaction networks has been used in yeast and mammals. In plants, many proteins also have been identified and characterized by Y2H analysis. In this presentation, we intend to demonstrate the applicability of a combination of VIGS and Y2H to the study of molecular mechanisms of the Q gene action, to the investigation of wheat disease resistance to tan spot caused by Pyrenophora tritici-repentis, and to the research of wheat scab caused by Fusarium graminearum. Using VIGS to verify the biological relevance of these interactors cloned through Y2H analysis of Q and Ptr ToxA is in progress. Finally, we also demonstrate here the use of VIGS in the study of wheat scab disease.