Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/20/2005
Publication Date: 1/14/2006
Citation: Phillips, R.L., Rines, H.W., Okagaki, R., Kynast, R., Stec, A.O., Jacobs, M.S., Galatowitsch, M., Huettl, P., Schmidt, C., Suresh, J., Baumgarten, A., Odland, W.O., Cabral, C., Walch, M., Kowles, R.V., Retzel, E. 2006. Availability and use of oat-maize addition and radiation hybrid lines [abstract]. Plant and Animal Genome XIV Abstracts. p. 192. Interpretive Summary:
Technical Abstract: Creation of oat-maize addition lines (OMAs) by alien introgression of individual maize (Zea mays) chromosomes into the hexaploid oat (Avena sativa) genome reduces maize genome complexity 10-fold. OMAs allow for mapping of sequences to chromosome without polymorphism and validate chromosomal allocation of genes, markers, and BACs. Disomic OMAs have been recovered for all chromosomes in a Seneca60 maize background. When combined, DNAs for a complete set of OMAs derived from maize inbreds B73 and Mo17 are available and a complete set for each inbred is being pursued. Irradiation of monosomic OMA seeds produces radiation hybrids (RH) which carry deletions and intergenomic translocations. Uses for RHs are expanding beyond as a high-throughput mapping tool via presence/absence assays. RH mapping panels anchored to existing genetic maps provide a means to: physically map genes more precisely, aid in assembling contigs and BAC-end sequences, survey the distribution and concentration of repetitive and low-copy sequences along a chromosome, identify gene rich and poor areas of a chromosome, identify chromosomal segments containing candidate genes for cloning and manipulation, position centromeres on genetic maps and define them to very small regions, and allow assignment of duplicated genes and members of multigene families to identify paralogous segments. OMA and RH lines represent a unique opportunity to study intergenomic gene interaction, evolution, expression, physiology, chromosome pairing, and recombination. DNA or seed for the available Seneca60, B73, and Mo17 OMAs and RH lines are ready for distribution. This work is supported by the National Science Foundation under Award No. 0110134.