Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2005
Publication Date: 1/14/2006
Citation: Phillips, R.L., Rines, H.W., Okagaki, R.J., Kynast, R., Odland, W.E., Cabral, C.B., Walch, M.D., Baumgarten, A.M., Stec, A.O., Jacobs, M.S., Galatowitsch, M.W., Huettl, P.A., Schmidt, C.M., Suresh, J., Kowles, R.V., Retzel, E. 2006. Chromosome-by-chromosome maize genome analysis using oat-maize addition lines [abstract]. Plant and Animal Genome XIV Abstracts. p. 78. Interpretive Summary:
Technical Abstract: Oat-maize Addition lines (OMA 1 through 10) are available where one maize chromosome has been individually added to the oat genome by a wide cross. By gamma irradiation, several hundred Radiation Hybrid (RH) lines now exist with only a fragment of the maize chromosome resulting from deletions of the maize chromosome or translocations to an oat chromosome. The total number of RH lines recovered follows: chromosome 1 (201), 2 (81), 3 (39), 4 (71), 5 (6), 6 (121), 7 (13), 8 (0), 9 (202), 10 (2). The RH lines provide mapping panels for both low- and medium-resolution physical mapping and for contig assembly and map-based cloning. Most lines have maize chromosomes from Seneca 60; however, when combined, DNA for a complete set is now available with B73 or Mo17 backgrounds. Derived telosomic and truncated chromosomes with only a short pericentromeric segment have been used to physically map six maize centromeres. Gene family members can be readily mapped without the need for polymorphisms. Evolutionary information from the chromosome-by-chromosome distribution of repetitive sequences by microarray analysis indicates macro-duplications similar to those determined by low-copy sequences. OMAs and RHs provide unique opportunities to study intergenomic gene interaction, expression and silencing, chromosome pairing, and other important genetic phenomena such as C4 photosynthetic metabolism. Two key enzymes in C4 metabolism, phosphoenolpyruvate carboxylase (PEPc) and pyruvate orthophosphate dikinase (PPDK), express only in OMA 9 and OMA 6, respectively, as expected from previous mapping information.