|Chase, Chadwick - Chad|
Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/14/2006
Publication Date: 1/14/2006
Citation: Chase, C.C., Mariasegaram, H., Chaparro, J.X., Olson, T.A., Brenneman, R.A., Niedz, R.P. 2006. Mapping the locus causing the slick phenotype in senepol derived cattle [abstract]. Annual International Plant & Animal Genome Conference. January 14-18, 2006, San Diego, CA. p. 232. Interpretive Summary:
Technical Abstract: The ability to maintain normal rectal temperatures during heat stress is an important attribute for cattle in the subtropics and tropics. Previous studies have shown that Senepol cattle and their crosses are as heat tolerant as Brahman cattle. This has been attributed to the slick hair coat of Senepol cattle, which is thought to be controlled by a single dominant gene. This study represents the first attempt to map the gene responsible for the slick phenotype in cattle. A rapid genome scan using the bulked segregant analysis (DNA pooling) was conducted to identify the chromosomal region harboring the slick gene. Separate DNA pools were constructed to bulk the slick (n=10) and normal hair coat half-sib daughters (n=10) sired by a heterozygous Senepol bull out of Holstein dams. The sire and the DNA pools were genotyped with 340 microsatellite markers. The peak heights in the electropherograms were used to analyze the data. Of the 340 markers used in the initial genome scan, 177 proved informative. Some (118) were homozygous in the sire and therefore deemed uninformative, while others failed to amplify (45). A total of 23 markers showed evidence of linkage (P<0.05) to the slick phenotype and were distributed over 12 different bovine chromosomes. Further analysis indicated that most were false positives, and only one region in chromosome, BTA 20, was selected as a candidate based on accumulation of highly significant evidence at three adjacent markers (BMS2461, BM4107, and BMS703 at 33.4, 57.3, and 60.1 cM, respectively, P<0.0001).