Submitted to: Genbank
Publication Type: Abstract Only
Publication Acceptance Date: 9/21/2005
Publication Date: 10/11/2005
Citation: Proszkowiec-Weglarz, M. Richards, M.P., Poch, S.M. 2005. Gallus gallus 5'-AMP-activated protein kinase, gamma-2 non-catalytic subunit, transcript variant 2 (PRKAG2) mRNA, complete cds, alternatively spliced. GenBank Accession No. DQ212709.
Technical Abstract: AMP-activated protein kinase (AMPK) serves a ‘fuel gauge’ that responds to fluctuations in cellular energy level. AMPK exists as an enzyme complex consisting of one catalytic subunit (alpha) and two regulatory subunits (beta and gamma). A molecular cloning strategy involving primer-directed reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of 5’ cDNA ends (5’ RACE) was developed to sequence 2448 base pairs (bp) of a chicken heart–derived cDNA corresponding to the complete coding region and portions of the 5’- and 3’-untranslated regions of the chicken AMPK gamma-2 mRNA transcript variant 2. As compared to transcript variant 1, choosing an alternative initiation and promoter site in the AMPK gamma-2 gene caused the loss of first three exons and the inclusion of the alternative first exon. The chicken AMPK gamma-2 transcript variant 2 mRNA codes for a predicted 452 amino acid protein which contains four cystathionine beta-synthase (CBS) domains (two Bateman domains). This sequence data confirmed for the first time the existence and expression of the AMPK gamma-2 variant 2 mRNA in chickens. Such data will be useful in studying the structure and expression of the chicken AMPK gamma-2 gene, as well as, in further defining the function of the AMPK pathway in poultry.