Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/3/2005
Publication Date: 9/15/2005
Citation: Chitko McKown, C.G., McKown, R.D., Laegreid, W.W. 2005. Expression of bovine PTX3 [abstract]. Journal of Leukocyte Biology (Suppl. 2005 Addendum). No. LB20 (98).
Technical Abstract: Bovine PTX3 was first identified in a study measuring changes in macrophage gene expression in response to E. coli O157:H7 LPS. We used human gene sequence information and our normalized EST libraries developed from pooled bovine tissues to identify homologous bovine sequences which were then used to design bovine-specific real-time PCR primers. Amplicons resulting from the use of these primers were sequenced and the products were shown to be specific for bovine PTX3. Additional information that has become available from the bovine genome sequencing project has shown sequence similarity between human and bovine PTX3 to be approximately 90% at both the nucleotide and protein levels. Experiments were performed using real-time PCR to determine the onset and duration of PTX3 mRNA expression in bovine PBMC when exposed to bacterial cell wall products. When treated with LPS, bovine monocytes and adherent PBMC showed a sharp increase in PTX3 mRNA abundance at 4-8H which declined by 24H and then increased at 48H. Non-adherent PBMC showed no increase in PTX3 mRNA until 48H. Additionally, at 48H, adherent PBMC showed a marked increase in PTX3 expression in response to exposure to peptidoglycan. Results generated from these experiments will increase our understanding of the role of PTX3 in the bovine innate immune response.