Submitted to: American Society for Microbiology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/30/1999
Publication Date: N/A
Citation: Zhao, T., Zhao, P., Doyle, M.P., Cray, P.J., Ladely, S.R. 1999. Prevalence of salmonella typhimurium dt104 in retail ground beef. American Society for Microbiology Meeting. Session No. 128/P. Abstract P-50. P. 521.
Technical Abstract: Surveillance data of cattle and human isolates of Salmonella typhimurium DT104 indicate that this pathogen emerged much more rapidly than anticipated. Studies were done to determine the prevalence of DT 104 in ground beef with a total of 404 samples obtained at retail stores from New York, San Francisco, Philadelphia, Denver, Atlanta, Houston, and Chicago. Salmonella spp. were isolated from fourteen (3.5%) samples. Eight different serotypes were identified among the isolates, including S. typhimurium (5), S. lille (3), S. Montevideo (1), S. hadar (1), S. meleagidis (1), S. cerro (1), S. Kentucky (1) and S. muenster (1). Drug-resistance assays revealed to ampicillin, streptomycin, sulphamethoxazole, and tetracycline, but sensitive to chloramphenicol. Phage typing revealed that all five S. typhimurium isolates were DT104A. All five DT 104 isolates were obtained from ground beef sampled from retail outlets in San Fransisco. PFGE genomic DNA profiles of the five DT104 isolates from ground beef were identical to those of four DT104 isolates from cattle.