BACTERIOCINS REDUCE CAMPYLOBACTER COLONIZATION AND ALTER GUT ARCHITECTURE IN TURKEY POULTS

Authors: COLE, KIMBERLY - UNIV OF ARKANSAS; FARNELL, MORGAN - 6226-05-00; DONOGHUE, ANN - 6226-05-00; Stern, Norman; SVETOCH, E - CTR FOR APP. MICRO; ERUSLANOV, B - CTR FOR APP. MICRO; KOVALEV, Y - CTR FOR APP. MICRO; PERELYGIN, V - CTR FOR APP. MICRO; LEVCHUCK, V - CTR FOR APP. MICRO; REYES-HERRERA, IXCHEL - UNIV OF ARKANSAS

Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2005
Publication Date: 9/6/2005
Citation: 2005. Bacteriocins reduce campylobacter colonization and alter gut architecture in turkey poults. Campylobacter Helicobacter and Related Organisms International Workshop.

Interpretive Summary:

Technical Abstract: Campylobacter is a leading bacterial cause of human foodborne illness. Recent evidence has demonstrated that bacteriocins produced by Lactobacillus and Paenibacillus bacteria inhibit C. jejuni growth in vitro and colonization in vivo, in broiler chicks. Campylobacter coli is the most prevalent Campylobacter isolate found in turkeys. The objective of the present study was to evaluate the efficacy of bacteriocins against C. coli colonization and to evaluate the influence of bacteriocins on the gastrointestinal architecture in poults. In three separate trials, day-of-hatch turkey poults were randomly allocated to one of three treatment groups (total 90 poults; 30/treatment). Three days post-hatch, poults in each treatment group were orally challenged with approximately 1x106 cfu of C. coli. On days 10-12 post-hatch, the control group had free access to a commercial diet, and the two different bacteriocin treatment groups were fed the same diet supplemented with purified, microencapsulated bacteriocins (500 mg per kg of feed). On day 13 post-hatch (3 days bacteriocin treatment), ceca were aseptically removed for enumeration of Campylobacter and duodenal loops were collected for morphometric analysis. Campylobacter was undetectable in poults supplemented with bacteriocins in all trials; whereas untreated controls were colonized with 5-6 log10 cfu per g of cecal contents. Duodenum crypt depth was decreased in poults treated with either bacteriocin compared to controls (P<0.05). Intestinal crypts are an important niche for Campylobacter colonization in poultry. The dynamic reduction in crypt depth in poults supplemented with bacteriocin may provide clues to how bacteriocins inhibit Campylobacter in vivo.