Submitted to: Meeting Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/1/2004
Publication Date: 5/1/2004
Citation: Rijnkels, M. 2004. Functional genomics of the caseins. Meeting Proceedings of the 18th Annual J.R. Brunner Symposium at Michigan State University, East Lansing, MI (5/20-21/2004). Interpretive Summary: Interpretive Summary not needed for this 115.
Technical Abstract: We are using genomic approaches to study the caseins and their transcriptional regulation. The casein gene cluster consists of three (or four depending on the species) evolutionarily related genes and one physically and functionally linked gene. Evolutionary conserved sequences were identified in the genomic domain harboring the casein gene cluster by comparative sequence analysis in 5-7 mammalian species. Working from the assumption that evolutionary conservation is an indicator for functionality this approach was used to study the casein genes in different species and learn more about the divergence and differences in caseins between species. We also were able to identify a number of non-casein genes in the genomic domain encompassing the caseins and study their relationship to the caseins. Additionally non-coding evolutionary conserved regions (n-ECRs) might point to a functional role in gene regulation. The potential involvement of n-ECRs in the regulation of the casein gene cluster domain is studied by chromatin status analysis, transgenic and computational approaches.