Submitted to: Euphytica
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/16/2004
Publication Date: 1/31/2005
Citation: Gedye, K.R., Bettge, A.D., King, G.E., Morris, C.F. 2005. Evaluation of maternal parent and puroindoline allele on kernel texture in a reciprocal cross between two hard spring wheat cultivars. Euphytica 141:121-127. Interpretive Summary: Kernel softness is an important quality characteristic of wheat. Gene sequence variation and mutations to the two puroindoline genes account for the majority of variation in wheat kernel softness. Other factors also influence kernel softness, including effects associated with the maternal parent. A genetic population derived from a two hard wheat varieties was evaluated for kernel softness and puroindoline gene sequence. The maternal parent and puroindoline sequence both had effects on kernel softness.
Technical Abstract: Kernel texture is an important quality characteristic of wheat (Triticum aestivum L.). Gene sequence variation and mutations to the two puroindoline genes (Pina and Pinb), located at the Ha locus on chromosome 5DS, account for the majority of variation in wheat kernel texture. Other factors also influence kernel texture, including effects associated with different maternal parent. A population of recombinant inbred lines (RILs) derived from a reciprocal cross between two wheat cultivars ‘ID377s’ (Pina-D1b / Pinb-D1a) and ‘Klasic’ (Pina-D1a / Pinb-D1b) were examined in two succeeding generations. Kernel texture was determined using the Single Kernel Characterization System (SKCS) and the RIL puroindoline haplotype was identified by the sequence-specific PCR amplification of each gene. ANOVA identified a significant effect of the maternal parent and puroindoline mutation on kernel texture. RILs containing the Pina-D1b mutation were significantly harder than lines containing the Pinb-D1b mutation. RILs which had Klasic as the maternal parent were significantly harder than those which had ID377s as the maternal parent. When the maternal parent and puroindoline allele were analyzed in combination, RILs derived from Klasic as the maternal parent and the Pina-D1b allele were significantly harder than those containing the same allele but ID377s as the maternal parent. The same occurred for RILs containing the Pinb-D1b allele, lines with Klasic as the maternal parent were harder than lines with ID377s as the maternal parent. These results corroborate the harder phenotype of the Pina-D1b allele and indicate a significant maternally-inherited contribution to kernel texture variation.