Submitted to: Cytogenetics and Genome Research
Publication Type: Peer reviewed journal
Publication Acceptance Date: 6/20/2005
Publication Date: 12/20/2005
Citation: Shimogiri, T., Kiuchi, S., Hiraiwa, H., Hayashi, T., Takano, Y., Yoshizane, M., Rohrer, G.A., Milan, D., Yasue, H. Assignment of 204 genes localized on hsa17 to a porcine rh (imprh) map to generatate a dense comparative map between pig and human/mouse. Cytogenetics and Genome Research. 112:114-120. 2006. Interpretive Summary: Pig chromosome 12 has been shown to possess genes influencing economically important traits such as fatty acid composition, growth rate after weaning, and meat color. While pig chromosome 12 is known to contain genes that are located on human chromosome 17, many rearrangements in gene order are suspected and the current resolution is not sufficient for effective selection of candidate gene(s) responsible for these traits. Thus, a study to construct a high resolution comparative map between pig chromosome 12 and human chromosome 17 was designed. Over 200 genes that reside on human chromosome 17 spanning the entire chromosome were placed on pig chromosome 12. The resultant high resolution comparative map revealed numerous rearrangements occurred during the evolution of mammals for this chromosome. In addition, this map makes it easier to select positional candidate genes for growth and pork quality traits and should expedite the development of useful genetic markers for selection of superior pigs.
Technical Abstract: Bi- and uni-directional chromosome painting (ZOO-FISH) and gene mapping have revealed correspondences between human chromosome (HSA) 17 and porcine chromosome (SSC) 12 harboring economically important quantitative trait loci. In the present study, we have assigned 204 genes localized on HSA17 to SSC12 to generate a comprehensive comparative map between HSA17 and SSC12. Two hundred fifty-five primer pairs were designed using porcine sequences orthologous with human genes. Of the 255 primer pairs, 208 (81.6%) were used to assign the corresponding genes to porcine chromosomes using the INRA-Minnesota 7000-rad porcine × Chinese hamster whole genome radiation hybrid (IMpRH) panel. Two hundred three genes were integrated into the SSC12 IMpRH linkage maps; and one gene, PPARBP, was found to link to THRA1 located in SSC12 but not incorporated into the linkage maps. Three genes (GIT1, SLC25A11, and HT008) were suggested to link to SSC12 markers, and the remaining one (RPL26) did not link to any genes/expressed sequence tags/markers registered, including those in the present study. A comparison of the gene orders among SSC12, HSA17, and mouse chromosome 11 indicates that intra-chromosomal rearrangements occurred frequently in this ancestral mammalian chromosome during speciation.