Author
CASTRILLO, L - CORNELL UNIVERSITY | |
ANNIS, S - UNIVERSITY OF MAINE | |
GRODEN, E - UNIVERSITY OF MAINE | |
MISHRA, P - UNIVERSITY OF MAINE | |
Vandenberg, John |
Submitted to: Society for Invertebrate Pathology Annual Meeting
Publication Type: Abstract Only Publication Acceptance Date: 7/31/2005 Publication Date: 8/7/2005 Citation: Castrillo, L.A., Annis, S.L., Groden, E., Mishra, P.K., Vandenberg, J.D. 2005. Low likelihood of recombination between the introduced beauveria bassiana strain gha and indigenous conspecific strains based on vegetative compatibility groupings [abstract]. Society for Invertebrate Pathology Annual Meeting. 38:50. Interpretive Summary: Technical Abstract: Among fungi with no known sexual stage or are predominantly haploid, the parasexual cycle provides a means of DNA exchange and recombination as has been shown in a wide range of asexual fungi. During the parasexual cycle, vegetatively compatible hyphae fuse allowing exchange of genetic material and recombination. Genetic recombination is an important component of risk assessment studies of entomopathogens that are sprayed repeatedly in large quantities in agricultural fields as microbial control agents. The likelihood of recombination and the impact of resulting recombinants on non-target organisms need to be considered because strain virulence and host range are critical to assessing safety. We assessed the likelihood of recombination between the commercial strain Beauveria bassiana GHA and indigenous conspecific strains by determining vegetative compatibility groups present in fields in Maine and New York with various histories of GHA application. Thirty-seven strains out of 110 soil isolates characterized using AFLP and RAPD markers were selected to represent the different cluster groups of indigenous and GHA-similar genotypes observed. Results showed that strains from all three genetic clusters of indigenous populations found in Maine and New York were vegetatively incompatible with GHA, indicating low likelihood of parasexual recombination between the introduced and indigenous strains. |