Submitted to: Proceedings of the National Academy of Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/27/2005
Publication Date: 2/2/2005
Citation: Feil, H., Feil, W.S., Chain, P., Larimer, F., Dibartolo, G., Copeland, A., Lykidis, A., Trong, S., Nolan, M., Goltsman, E., Thiel, J., Malfatti, S., Loper, J.E., Lapidus, A., Detter, J.C., Land, M., Richardson, P.M., Kyrpides, N.C., Ivanova, N., Lindow, S.E. 2005. comparison of the complete genome sequences of pseudomonas syringae pv. syringae b728a and pv. tomato dc3000. Proceedings of the National Academy of Sciences. 102(31):11064-11069. Interpretive Summary: We sequenced the genome of the plant pathogenic bacterium Pseudomonas syringae pathovar syringae strain B728a, and compared the sequence to another strain of the same species. Because the two strains differ in the plants they attack and their abilities to live on plant surfaces, comparison of their sequences highlighted genes involved in these aspects of pathogenicity. Knowledge of the genomic sequence of different strains of important plant pathogens is invaluable to scientists studying pathogenicity and is already being used to advance knowledge of plant pathogens for the benefit of agriculture.
Technical Abstract: he complete genome of Pseudomonas syringae pathovar syringae B728a (Pss B728a) was sequenced, and the sequence was compared with that of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The two pathovars of this economically important species of plant pathogenic bacteria differ in host range and other interactions with plants, with Pss having a more pronounced epiphytic stage of growth and higher abiotic stress tolerance and Pst DC3000 having a more pronounced apoplastic growth habitat. The Pss B728a genome (6.1 megabases) contains a circular chromosome and no plasmid, whereas the Pst DC3000 genome is 6.5 mbp in size, composed of a circular chromosome and two plasmids. While a high degree of similarity exists between the two sequenced Pseudomonads, 976 protein-encoding genes are unique to Pss B728a when compared to Pst DC3000, including large genomic islands likely to contribute to virulence and host specificity. Among the 976 genes of Pss B728a with no counterpart in Pst DC3000 are those encoding for syringopeptin (SP), syringomycin (SR), indole acetic acid biosynthesis, arginine degradation, and production of ice nuclei. The genomic comparison suggests that several unique genes for Pss B728a such as ectoine synthase, DNA repair, and antibiotic production may contribute to epiphytic fitness and stress tolerance of this organism.