Submitted to: Acta Horticulture Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 8/1/2005
Publication Date: 9/12/2005
Citation: Aradhya, M.K., Stover, E.W., Prins, B.H., Dangl, G.S. Use of genetic markers in grape at the national clonal germplasm repository in davis, california. Acta Horticulture Proceedings pp. 137-142. Interpretive Summary: This paper describes the use of DNA tests to evaluate diverse grapevines at the National Clonal Germplasm Repository (NCGR) in Davis, California which maintains most of the Mediterranean-adapted fruit and nut crop collections in the U.S. The NCGR missions are to acquire, preserve, evaluate and distribute the genetic diversity in our designated crops. The NCGR grape collection includes >2800 different types of grapevine, including an extensive collection of wild grape species, ~600 cultivated wine grape cultivars, and ~600 cultivated table grape cultivars. At the Davis NCGR, DNA tests have been used to test variability and relatedness within collections and trueness to type of material. To date, DNA tests have been used on 700 NCGR grapevine types, including over 400 cultivated grapes and ~300 wild grapes representing 44 species from both the New and Old World. The NCGR is committed to acquiring additional material, with a special interest in protecting collections which may otherwise be lost.
Technical Abstract: The Davis, California National Clonal Germplasm Repository (NCGR) houses most Mediterranean-adapted fruit and nut crop collections in the U.S., including grapes. The NCGR is part of the USDA National Plant Germplasm System (NPGS). Our missions are to acquire, preserve, characterize and distribute germplasm resources of our designated crops. It is NPGS policy to distribute plant material, free of charge, to research interests around the world (see http://www.ars-grin.gov/dav/). The NCGR grape collection includes >2800 different accessions, including one of the world’s most comprehensive Vitis species collections, ~600 V. vinifera wine grape cultivars, and ~600 V. vinifera table grape cultivars. Our sister repository in Geneva, NY houses the cold-hardy grape collection. At the Davis NCGR, genetic markers have been used to assess diversity, structure and differentiation within collections, and trueness to type of germplasm accessions. To date, Simple Sequence Repeats (SSRs) and Amplified Fragment Length Polymorphism (AFLP) markers have been used to analyze over 700 NCGR grape accessions, including over 400 V. vinifera and ~300 wild grapes representing 44 taxa from both the New and Old World. The paper provides a general description of grape collection and ongoing research projects on quantifying and describing the genetic diversity and patterns of distribution of within and between different gene pools.