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Title: USE OF A MULTIPLEX RT-PCR TO DIFFERENTIATE LENTOGENIC NDV FROM END

Author
item Sellers, Holly
item Linnemann, Erich
item Kapczynski, Darrell

Submitted to: American Association of Avian Pathologists
Publication Type: Abstract Only
Publication Acceptance Date: 2/8/2005
Publication Date: 7/16/2005
Citation: Sellers, H.S., Linnemann, E.G., Kapczynski, D.R. 2005. Use of a multiplex RT-PCR to differentiate lentogenic NDV from END [abstract]. American Association of Avian Pathologists. p. 32.

Interpretive Summary:

Technical Abstract: A multiplex RT-PCR has been designed to differentiate lentogenic Newcastle disease viruses from exotic Newcastle disease (END). Using computer sequence analysis and published sequences (GenBank), we have constructed lentogenic (B1/LaSota) and END (CA02)-specific primer sets. The primers amplify regions within the phosphoprotein and fusion protein genes of NDV. The primer sets have been tested in uniplex RT-PCR assays and amplify the predicted target templates. Our preliminary evaluation of the primers in a multiplex RT-PCR format suggests the primers will be effective in discriminating lentogenic and velogenic NDVs. The multiplex RT-PCR parameters have been optimized. RNA from archived NDV field isolates and END experimentally infected and uninfected chickens will be tested. The sensitivity and specificity is currently being determined