Submitted to: World Poultry Science Association Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/8/2005
Publication Date: 9/15/2005
Citation: Kapczynski, D.R. 2005. Sequence analysis of the toll-like receptor 7 from gallinaceous and non-gallinaceous species. World Poultry Science Association Proceedings. p. 73.
Technical Abstract: The immune system can be divided into two functional components, the innate and adaptive, that differ in their mechanism of pathogen recognition. The innate immune response is responsible for detecting invading microorganisms during the initial stages of infection, which is a crucial determinant of disease resistance or susceptibility. The innate system uses germ-line encoded receptors, termed pattern-recognizing receptors (PRR’s) to recognize highly conserved pathogen-associated molecular patterns (PAMP’s) from infectious agents. The Toll-like receptor (TLR) family members are membrane-bound PRR’s responsible for recognizing the presence of invading microorganisms through PAMP’s. Recently, several chicken TLR’s have been identified, including TLR7, which is reported to be involved with recognition of ribonucleic acid components characteristic of viral genomes (e.g. ssRNA). In this study, we amplified a 416 bp product of TLR7 using RT-PCR with mRNA derived from 13 different avian species. The nucleotide and predicted amino acid sequences were compared to previously reported TLR 7 sequences from different sources. Preliminary results indicate 99 % similarity between chicken and turkey species, and 64 or 70 % similarity to human and murine species, respectively. The phylogenetic relationship of various TLR7’s from avian and mammalian origin will be discussed.