Submitted to: North American Barley Research Workshop Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 6/15/2005
Publication Date: 7/17/2005
Citation: Muehlbauer, G.J., Garvin, D.F., Smith, K., Boddhu, J., Seung, C. 2005. Applications of genechips for barley improvement. North American Barley Research Workshop Proceedings, Red Deer, Alberta. p. 143-147. Interpretive Summary:
Technical Abstract: The use of RNA profiling has become a powerful strategy to examine genome-wide gene transcript accumulation patterns. The Barley1 Affymetrix GeneChip probe array is a resource that can be used to examine the expression of greater than 20,000 genes in parallel. This article summarizes several research projects that are using the Barley1 Affymetrix GeneChip for a diverse range of studies. This includes physically mapping large numbers of barley genes to individual chromosomes and chromosome arms in wheat-barley aneuploid stocks, examining RNA profiles in barley infected with Fusarium graminearum, and identifying markers for specific barley genome regions in a targeted manner. The recent development of the Barley1 GeneChip probe array has provided the barley genetics community a new high-throughput tool to assess gene expression patterns. Thus, the Barley1 GeneChip probe array provides the barley genetics community a new high-throughput tool to assess gene expression patterns for a variety of purposes.