Submitted to: Journal of Biomolecular Techniques
Publication Type: Abstract Only
Publication Acceptance Date: 5/31/2005
Publication Date: 6/20/2005
Citation: Thannhauser, T.W., Yang, Y., Xiang, B., Lu, S., Li, L. 2005. Differential protein expression in brassica oleracia: changes associated with the ability to accumulate high concentrations of b-carotene [abstract]. Journal of Biomolecular Techniques. 16:60.
Technical Abstract: The goal of this work is to study the differential protein expression between homozygous “white or wild-type” cauliflower (Brassica oleracea var. botrytis) (or/or), the homozygous orange colored mutant (Or/Or) and the orange colored heterozygote (Or/or). This study has been undertaken with a view towards developing an understanding of the processes associated with the ability to accumulate high levels of B-carotene, a phenotypic trait common to both Or/Or and Or/or and of considerable agronomic importance. Samples of curd tissues from field-grown plants were bulked according to genotype, the soluble proteins were extracted and analyzed by 2-Dimensional Difference Gel Electrophoresis (2D-DiGE). The greatest difference in protein expression was observed between or/or and Or/Or where the expression levels of 444 proteins were found to increase/decrease by an amount consistent with 95% probability of being real. This observation is consistent with the gross differences in phenotype that exist between Or/Or and or/or. A similar comparison of protein expression between or/or and Or/or found that only 56 proteins changed expression by a similar amounts. The 37 proteins common to both sets were excised from the gels, digested with trypsin and identified/characterized by tandem mass spectrometry. There is a high probability that these proteins are associated with B-carotene accumulation and knowledge of their identities and functions will provide a rough schematic of the pathways involved.