|Lay Jr, Donald|
Submitted to: American Society of Cell Biology Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 10/31/2005
Publication Date: 12/15/2005
Citation: Moulton, K., Williams, E., Lovell, F., Ryan, P., Lay Jr, D.C., Jansen, D., Willard, S. 2005. A comparison of optical clearing agents to enhance photonic detection of salmonella through pig skin. American Society of Cell Biology Proceedings.
Technical Abstract: The objective of this investigation was to evaluate glycerol (GLY), glucose (GLU), sucrose (SUC), and corn syrup (CS) as agents to optically clear pig skin and increase photonic transference and detection of photon emitting Salmonella typimurium (S. typh-lux; transformed with plasmid pAKnlux1), in a laboratory model for Salmonella detection in swine. Shoulder pig skin obtained after harvest was processed to remove hair and subcutaneous fat, and the skin measured for thickness. A 96-well plate containing S. typh-lux was imaged for five minutes using a photon counting camera (Berthold/Nightowl) as a control reference. Skin (2mm thick) was placed over the plate containing S. typh-lux and imaged for a five minute duration. The skin was then treated for four hours with varying ratios of GLY, GLU, SUC, and CS in a dose-dependent manner and the plate imaged again five minutes later. The percent of photonic emissions (treated or untreated skin relative to no skin controls) from three replicates within eight skin pieces per treatment were used for statistical analysis. Treatment for four hours with 100% CS increased (P<0.05) photonic emissions (21.6 +/- 2.0%) compared to untreated skin, 50% GLY and 50% SUC (0.6 +/- 0.2, 17.7 +/- 0.8 and 13.6 +/- 1.8% respectively). However 75% SUC and 50% SUC increased (P<0.05) photonic emissions (17.7 +/- 1.3 and 17.7 +/- 1.3% respecitively) compared to 75% GLU, 50% GLY and untreated skin (15.3 +/- 1.1, 15.4 +/- 0.8 and 1.1 +/- 0.1% respectively). These data indicate GLY, GLU, SUC, and corn syrup may be used as effective optical clearing agents on pig skin when treated for four hours to allow for an increased detection of emitted photons from S. typh-lux through skin. [USDA-NRI grant # 2003-35201-13841; USDA-ARS Biophotonic Initiative # 58-6402-3-0120].