Submitted to: Crop Science
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/31/2005
Publication Date: 10/27/2005
Citation: Panella, L.W., Lewellen, R.T. 2005. Registration of FC301, Monogerm, O-type Sugarbeet population resistant to Rhizomania and Cercospora Leaf Spot and moderately resistant to Black Root and Curly Top. Crop Science, 46(2):1009-1010. 2006. Interpretive Summary: FC301 is an O-type germplasm segregating for hypocotyl color and for monogerm seed. Two crosses were made. The first cross was 'C890'aa crossed with ' FC607' and 'FC604' and 'C859'aa crossed with ' FC607' and 'FC604'. Seed was combined, selfed families were produced, and they were selected for resistance to cercospora leaf spot (Fort Collins, CO) and Beet curly top virus (Kimberly, ID). They were then sent to Salinas where they were selected for rhizomania resistance, resistance to Erwinia root rot, powdery mildew, agronomic performance, and percent sucrose.
Technical Abstract: FC301 is an O-type germplasm segregating for hypocotyl color (94% red) and for monogerm (90%). Two crosses were made. The first cross was 'C890'aa x two pollen donors – ' FC607' and 'FC604'(approximately 50 F1 plants) bulked with the cross 'C859'aa x the same two pollen donors (approximately 50 F1 plants). F1 seed was combined for bulk increase of the F2 after germination testing to make the parental contribution equal from both female parents. 90 F2 mother roots were harvested and selfed. 75 selfed families were produced selected for resistance to cercospora leaf spot (Fort Collins, CO), and Beet curly top virus (Kimberly, ID). The best performing families in both nurseries were increased and seed was sent to Salinas, where selections were made for rhizomania resistance, resistance to Erwinia root rot, powdery mildew, agronomic performance, and percent sucrose. The selected roots were inter-pollinated, and monogerm and multigerm seed was separated forming two populations 99-1,2,3 M and 99-1,2,3 m. Seed from the monogerm populations was either sent to Oregon for steckling production or planted in the Salinas rhizomania nursery. Stecklings from male-fertile, high quality monogerm plants were selected and individually selfed to produce S1 progeny, and crossed to an annual male-sterile tester. The F1 hybrids were indexed for O-type in December, 2000, and found to be uniformly male-sterile, suggesting that O-type selection was unnecessary. Seed of the population and the S1 progenies was planted the Oregon steckling nursery and the Salinas rhizomania nursery. From the Salinas rhizomania nursery, S1 plants from within S1 progenies and plants from the population were selected for resistance to rhizomania. Concurrently, seed from the original Fort Collins population, which had been selected strictly for leaf spot resistance and then re-selected from leaf spot resistance using the leaf disc method, was planted also in the Salinas rhizomania nursery and Oregon steckling nursery. In March 2001, induced, selected plants from Salinas and stecklings form Oregon were pooled and recombined through the male-sterile plants from all three phases. There was nearly equal representation from the new Fort Collins cercospora leaf spot population, the S1 lines, and the population selected for rhizomania resistance.