Submitted to: American Society of Agronomy Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/10/2005
Publication Date: 11/10/2005
Citation: Ortiz-Perez, E., Cianzio, S., Wiley, H., Horner, H.T., Davis, W.H., Palmer, R.G. 2005. Evaluation of insect-mediated cross-pollinated hybrid soybean [Glycine max (l.) merr.]. American Society of Agronomy Abstracts. Abstract No. 406b. Interpretive Summary:
Technical Abstract: Manual cross-pollination to produce large quantities of hybrid soybean seed for yield trials is very difficult and time-consuming. As a result, parental combinations that produce heterosis levels superior to the best pure-line cultivars, (i.e., identification of heterotic patterns) have not been identified in soybean. However, insect-mediated cross-pollination has been shown to produce large quantities of hybrid soybean seed. The objective of this study was to evaluate F1 hybrid soybean seed from single–crosses, three-way crosses, and backcrosses (BC1F1) produced by selected male-sterile lines as female parents and a selected group of male parents. Bees were the pollinator vector. Eight cross-combinations were produced in a complete randomized block design with five replications in summer 2002. In summer 2003, the F1 seed and their parental lines were evaluated for yield, height, lodging, seed protein, seed oil and seed fatty acids content at two locations. In summer 2003, the BC1F1 and three-way crosses were produced. The BC1F1 seed was obtained from the plants produced from the original eight cross-combinations as female parents backcrossed to their recurrent male parents. The three-way seeds were produced from the original eight cross-combinations as female parents and crossed to a common male parent (a high yielding commercial line). The BC1F1, the three-way crosses, and their parental lines were evaluated in summer 2004. The results observed showed that for the traits evaluated, positive and negative mid-parent heterosis was present in the single-crosses, in the BC1F1 and in three-way crosses.