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ARS Home » Research » Publications at this Location » Publication #180913
Title: THE ANALYSIS OF STRUCTURED TRIGLYCERIDES, SYNTHESIZED AND IN COMMERCIAL FORMULATIONS, BY SILVER-ION HPLC

Author
item Adlof, Richard
item EMKEN, EDWARD - RETIRED ARS SCIENTIST
item List, Gary

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 6/15/2005
Publication Date: 8/28/2005
Citation: Adlof, R.O., Emken, E., List, G.R. 2005. The analysis of structured triglycerides, synthesized and in commercial formulations, by silver-ion HPLC [abstract]. American Chemical Society National Meeting. pp. 224.

Interpretive Summary:

Technical Abstract: The amounts and types of triacylglycerols (TAGs) in the oil phases of margarines and spreads are considered responsible for such properties as spreadability, resistance to water/oil loss and melting at body temperature. Recently, economic, health and consumer-driven factors have stimulated research to reduce the levels of trans fatty acids in lipid formulations. A method to analyze complex TAG mixtures was developed as part of a study relating the physical and sensory properties of reformulated spreads and margarines with the amounts/structures of their component TAGs. Silver-ion HPLC (Ag HPLC), utilizing silver ion-containing columns, was found to be a powerful technology for separation/quantization (“fingerprinting”) of synthesized TAG isomers and TAG formulations by fatty acid unsaturation (number, location, or configuration), chain length and/or location (1,2- vs. 3-position) on the TAG glycerol backbone, reproducible and more rapid than lipolysis/gas chromatography for analysis of TAG isomers. Specific examples are included.