Submitted to: American Society of Enology and Viticulture Annual Meeting Proceedings
Publication Type: Abstract only
Publication Acceptance Date: 5/11/2005
Publication Date: 7/12/2005
Citation: Huang, H., Lu, J., Ren, Z., Hunter, W.B., Dang, P.M. 2005. A comparative genomics analysis between V. shuttleworthii and V. vinifera grapes [abstract]. American Society of Enology and Viticulture Annual Meeting Proceedings. Interpretive Summary:
Technical Abstract: Vitis shuttleworthii, is a grape species native to the southeastern United States, which is known for its resistance to major grape diseases and pests. To identify and isolate the grape disease resistance genes, a V. shuttleworthii clone was chosen for EST analysis. In phase I of the EST project, a total of 12,936 ESTs were generated from a cDNA library derived from leaves and flowers during anthesis. Of which, 12,008 high quality ESTs and 5,766 unigenes were obtained. Among the 5,766 V. shuttleworthii unigenes, 157 have full length protein sequences. These 157 contigs with putative full length proteins were blasted against the V. vinifera unigenes set (a total of 23,871 unigenes generated from 139,380 ESTs, TIGR), and 153 of them had positive hits (E value 10-3). When the 5,766 V. shuttleworthii unigenes were compared to the 23,871 V. vinifera unigenes (BLASTN), 1,588 did not find significant matches (E value 10-1), and 1,086 of them did not find homologous protein sequences (E value 10-1). However, when the1,086 unigenes were blasted (BLASTX) against the 119,971 Arabidopsis protein database, only 428 did not have significant match (E value 10-2). When a set of 6,655 Arabidopsis protein sequences homologous to the V. shuttleworthii ‘unigue genes’ were blasted again V. viniferea unigenes, to our surprise, none of them were found homologous to the 23,871 V. viniferea unigenes. The potential usefulness of these “unique genes” and their role for disease resistance have been further verified by microarray analysis.