Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 7/1/2005
Publication Date: 6/1/2006
Citation: Hartung, J.S., Montera-Astua, M., Aguila, E., Chacon, C., Rivera, C. 2006. Molecular comparison of xylella fastidiosa isolates from costa rica, north and south america. American Phytopathological Society. 96(6):S164.
Technical Abstract: Diseases caused by Xylella fastidiosa have been described as emerging problems and serious threats to grapevine production in North America and to sweet orange production in Brazil. Recently, X. fastidiosa has been detected and isolated from grapevines, coffee and citrus plants in Costa Rica. Numerous genetic analyses of local X. fastidiosa populations have been done previously using several techniques including RAPDs and repetitive element PCR. However no Costa Rican isolates have been included and problems with reproducibility of RAPDs have been recognized. The variable number tandem repeats-PCR technique (VNTRs) has been reported to be a sensitive tool to detect variation among X. fastidiosa strains from a single host. A set of X. fastidiosa isolates from Costa Rica, Brazil and the USA were analyzed by VNTRs. Nine primer pairs for specific short sequence repeat (SSR) loci were used. Three of the SSR primer pairs either failed to render any product or amplification was inconsistent. Amplification of specific SSR loci was useful to distinguish among strains from different geographic origins and/or from different hosts. Variability was detected within North, Central and South American populations. The data will make it possible to determine the relationship between the Costa Rican strains and those from South and North America.