Submitted to: Sugar Journal
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2005
Publication Date: 6/1/2005
Citation: Comstock, J.C., Sood, S. and Mccorkle, K. Characterization of sugarcane populations for disease reactions for use in molecular marker research. Sugar Journal. 68:19. 2005
Technical Abstract: Molecular markers associated with resistance have been used for genotypic selection of individuals in a number of crops and may offer a means of detecting resistance in sugarcane. To initiate research and to associate molecular markers with resistance, several sugarcane populations were characterized for their reaction to yellow leaf, rust and ratoon stunt pathogens. The objective was to find populations with individuals that had extremes in disease reaction for yellow leaf, brown rust and ratoon stunt. Yellow leaf and rust data were taken from clones based on their natural infection. Ratoon stunt data were obtained from inoculated tests based on the number of Colonized Vascular Bundles. The mid-rib tissue immunoassay was used to detect Sugarcane Yellow Leaf Virus (SCYLV) in the phloem cells. Sixty-eight progeny of a cross of Green German x Ind 81-146 had been naturally exposed to SCYLV at Canal Point for 15 years. during a period where almost all other clones at Canal Point became infected within a period of 4-5 years. Thirty-nine clones of this population remained free of SCYLV for over the last three years. However, almost all other clones at Canal Point became infected within a period of 4-5 years. Rust data were taken from individuals from two families: 1) Green German x Ind 81-146 with 23 resistant progeny with no sporulating pustules and 165 susceptible progeny with varing amounts of sporulating pustules and 2) CP 94-1200 x CP 92-1167 with 33 resistant and 72 susceptible progeny. Progeny of six families were evaluated for their ratoon stunt reaction: Green German x Ind 81-146; Green German x SES 208; Green German x Coimbatore; CP 80-1827 selfed; SS 57-3 x Yacheng #12 and CP 94-1200 x CP 92-1167. All families exhibited extremes in disease reaction of individuals to ratoon stunt except that of Green German x Ind 81-146 population where all individuals were susceptible. Disease reaction data for the different populations and preliminary marker data will also be presented.