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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Grain Quality and Structure Research » Research » Publications at this Location » Publication #179980


item Tilley, Michael - Mike

Submitted to: Meeting Abstract
Publication Type: Other
Publication Acceptance Date: 4/22/2005
Publication Date: 5/22/2005
Citation: Tilley, M. 2005. Glucose oxidase effects on wheat flour albumins and gliadins. Program Book of the 3rd International Wheat Quality Conference. Meeting Abstract. p. 392

Interpretive Summary:

Technical Abstract: Chemical oxidants are routinely added to flour to modify rheological properties (shorten mixing time, improve gas retention, lower energy requirement for dough mixing) and enhance breadmaking performance (increase loaf volume and improve crumb structure). The elimination of potassium bromate, and possibly other chemical oxidant additives, presents a challenge to the baking industry. Alternative oxidation methods need to be found since industrial baking has been standardized with bromate. Substitution of chemical oxidants with enzymes is a desirable approach because enzymatic reactions are very specific, with little or no reactivity outside of the substrate. Oxidoreducing enzymes such as glucose oxidase (GOX) have been proposed as improvers for the baking industry. The mechanism of improvements caused by GOX is not understood. Following mixing wheat flour with and without the addition of GOX the different protein classes were extracted and analyzed by electrophoresis and size-exclusion HPLC. The most significant effects were observed to occur in the albumin (water-soluble) and gliadin (alcohol-soluble) protein groups. A significant increase in protein concentration and molecular weight distribution was observed in the albumin fraction by SE-HPLC. Further analysis revealed that this is due to changes in gliadin solubility. Gliadins are generally not soluble in water, however the inclusion of GOX in mixing renders the gliadins more water-soluble. The biochemical interactions responsible for this behavior and the possible effects on end-use properties are currently under investigation.