Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/2/2005
Publication Date: 7/12/2005
Citation: Lin, H., Takahashi, Y., Doddapaneni, H., Walker, A. 2005. Genome-wide comparative analysis of differentially regulated grape transcriptome in response to xylella fastidiosa infection. [Abstract]. International Grape Genomics Symposium. p.43
Technical Abstract: The gram-negative bacterium Xylella fastidiosa is the causal agent of Pierce’s disease (PD) in grape as well as diseases of many fruit and ornamental plants. A pair of PD resistant and susceptible sibling genotypes (V. rupestris x V. arizonica) was used in this comparative study. A total of 5,700 sequences were obtained from leaf, stem and shoot tissue specific subtractive and suppression hybridization cDNA libraries. Of these, 754 Expressed Sequence Tags (ESTs) showed BLASTX analysis (E=<10-5) and 439 ESTs showed (E=10-4-0.009) match to NCBI databases and the rest ~32 % of the sequences have not been previously described. The differentially expressed ESTs have been functionally grouped into defense, stress response, protein synthesis, signaling, and primary metabolic classes. A comparative analysis to identify up and down-regulated genes was made between infected and non-infected PD resistant and PD susceptible grapes. Ten candidate genes, associated with host/pathogen interactions, were further selected for Taq-Man gene expression analysis. Currently we are designing genome-wide 60-mer oligonucelotide microarrays to dissect spatial and temporal differential expression. This approach will help define the transcriptional pathways involved in host susceptibility and resistance in grape.