|Mcgrath, J Mitchell - Mitch|
Submitted to: Annual Beet Sugar Development Foundation Research Report
Publication Type: Research Notes
Publication Acceptance Date: 5/6/2005
Publication Date: 6/1/2005
Citation: Trebbi, D., McGrath, J.M. 2005. Phenotypic and genotypic analyses of root sucrose accumulation during juvenile to adult developmental phase change in sugar beet. 2004 Annual Beet Sugar Development Foundation Research Report. p. D11-D34.
Technical Abstract: The objectives of this research were to understand the dynamic of root sucrose content during the early root developmental phases in sugar beet, to characterize gene expression profiles during these early developmental phases, and to identify genes differentially expressed between developmental phases critical for sucrose accumulation. Identification of genes differentially expressed between developmental phases critical for sucrose accumulation was performed using Beta vulgaris cDNA microarray selected by subtractive hybridization of a sugar beet root developmental cDNA library and Arabidopsis thaliana cDNA and oligonucleotides microarrays. We identified critical root developmental stages for sucrose accumulation that could be used as selective indicator during breeding programs; detected overall gene expression changes during root development indicating possible timing of shift from juvenile to adult vegetative phases, related these changes with the phenotypic changes observed at the root system level; and identified genes possibly responsible for these changes. Results increase our understanding of metabolic pathways that regulate the early stages of root development, and sucrose accumulation in particular, in sugar beet.