Submitted to: American Phytopathological Society
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2005
Publication Date: 4/1/2005
Citation: DNA recombination/repair SMC family protein in phytoplasma. 2005. Phytopathology. 95:S50.
Technical Abstract: From the genome of clover phyllody (CPh) phytoplasma, a member of group 16SrI (aster yellows phytoplasma group), subgroup C, we cloned and determined the nucleotide sequence of a 2.8 kbp DNA fragment containing a gene encoding a putative protein related to eukaryotic tropomyosin. This gene was flanked at the 5’-end by a sequence encoding a partial protein similar to FtsH-related proteins of onion yellows (OY-M) (subgroup 16SrI-B) and Australian tomato big bud (group 16SrII) phytoplasmas and at the 3’-end by a nptA (Na+/phosphate symporter)-like gene and a partial hypothetical protein gene similar to PAM577 of onion yellows phytoplasma. The deduced structure of the myosin-like protein includes two internal repeat regions, a tropomyosin-like domain, and a coiled coil region. Comparative analyses indicated this protein is similar to eukaryotic DNA recombination/repair SMC (for Structural Maintenance of Chromosome) family proteins. NCBI BLAST searches of the OY-M complete genome sequence and of the nearly completed genome sequence of Spiroplasma kunkelii failed to reveal evidence for a similar protein encoded by these organisms. However, the CPh tropomyosin-like protein shared low similarity with adhesion–related proteins in mycoplasmas. It would be interesting to know whether this protein plays any role in host adaptation and/or pathogenicity of phytoplasmas.