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ARS Home » Southeast Area » Miami, Florida » Subtropical Horticulture Research » Research » Publications at this Location » Publication #178834


item Meerow, Alan
item Roh, Mark
item Schnell Ii, Raymond

Submitted to: Molecular Ecology Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/28/2005
Publication Date: 1/27/2006
Citation: Meerow, A.W., Roh, M.S., Schnell II, R.J. 2006. Sixteen microsatellite loci from Halesia tetraptera (Styracaceae). Molecular Ecology Notes. 5:777-779

Interpretive Summary: We have developed 16 microsatellite DNA markers for the Carolina silverbell, an undertsory tree of the southeastern U. S. hardwood forests. These markers are able to distinguish very closely related individuals and will be used to characterize a large germplasm collection of this species established by the U. S. National Arboretum.

Technical Abstract: Sixteen microsatellite loci isolated from Halesia tetraptera are described. All 16 are polymorphic, with four to 13 alleles across 15-19 members of a single half-sib family. Heterozygosity ranged from 0.067 to 1. One locus departs significantly from Hardy Weinberg Equilibrium in our test family. The test population shows significant heterozygote deficiency at this and two other loci. Thirteen loci exhibit significant linkage disequilibrium. These loci will be utilized in paternity analyses of geographically diverse half-sib families.