Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/23/2005
Publication Date: 7/16/2005
Citation: Lin, H., Walker, A.M., Takahashi, Y., Doddapaneni, H. 2005. Comparative analysis of differentially regulated expression of grapes in response to xylella fastidiosa infection. [Abstract]. American Society of Plant Biologists Annual Meeting. p.165. Interpretive Summary:
Technical Abstract: The gram-negative bacterium Xylella fastidiosa is the causal agent of Pierce’s disease (PD) in grape as well as diseases of many fruit and ornamental plants. A pair of PD resistant and susceptible sibling genotypes (V. rupestris x V. arizonica) was used for a comparative study. Analysis of 5,700 sequences derived from 12 tissue specific suppression and subtractive hybridization cDNA libraries found the sequences corresponded to 1,024 contigs. Sixty-seven percent of the contigs matched to NCBI databases based on BLASTX analysis and 32% have not been previously described. We identified putative genes that were expressed differentially in response to Xf challenge in resistant and susceptible leaf, stem and shoot tissues. We categorized expression profiles into functional classes related to defense, stress responses, protein synthesis, signaling, and primary metabolism. Ten candidate genes, associated with host/pathogen interactions, were selected for Taq-Man gene expression analysis. To further dissect spatial and temporal differential expression, we are designing 60-mer oligonucelotide microarrays for global expression analysis. This approach will help reveal the transcriptional pathways involved host susceptibility and resistance in grape.