Skip to main content
ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #178350
Title: GENOME SEQUENCING AND ANALYSIS OF ASPERGILLUS ORYZAE

Author
item MACHIDA, MASAYUKI
item ASAI, KIYOSHI
item SANO, MOTOAKI
item TANAKA, TOSHIHIRO
item KUMAGAI, TOSHITAKA
item TERAI, GORO
item KUSUMOTO, KEN-ICHI
item ARIMA, TOSHIHIDE
item AKITA, OSAMU
item KASHIWAGI, YUTAKA
item ABE, KEIETSU
item GOMI, KATSUYA
item HORIUCHI, HIROYUKI
item KITAMOTO, KATSUHIKO
item KOBAYASHI, TETSUO
item TAKEUCHI, MICHIO
item DENNING, DAVID
item GALAGAN, JAMES
item NIERMAN, WILLIAM
item Yu, Jiujiang
item ARCHER, DAVID
item Bhatnagar, Deepak
item Cleveland, Thomas
item GOTOH, OSAMU
item HORIKAWA, HIROSHI
item HOSOYAMA, AKIRA
item ICHINOMIYA, MASAYUKI
item IGARASHI, RIE
item IWASHITA, KAZUHIRO
item JUVVADI, PRAVEEN RAO
item KATO, MASASHI
item KATO, YUMIKO
item KIN, TAISHIN
item KOKUBUN, AKIRA
item MAEDA, HIROSHI
item MAEYAMA, NORIKO
item MARUYAMA, JUN-ICHI
item NAGASAKI, HIDEKI
item NAKAJIMA, TASUKU
item ODA, KEN
item OKADA, KINYA
item PAULSEN, IAN
item SAKAMOTO, KAZUTOSHI
item SAWANO, TOSHIHIKO
item TAKAHASHI, MIKIO
item TAKASE, KUMIKO
item TERABAYASHI, TASUNORI
item WORTMAN, JENNIFER
item YAMADA, OSAMU
item YAMAGATA, OUHEI
item ANAZAWA, HIDEHARU
item HATA, YOJI
item KOIDE, YOSHINAO
item KOMORI, TAKASHI
item KOYAMA, YASUJI
item MINETOKI, TOSHITAKA
item SUHARNAN, SIVASUNDARAM
item TANAKA, AKIMITSU
item ISONO, KATSUMI
item KUHARA, SATORU
item OGASAWARA, NAOTAKE
item KIKUCHI, HISASHI

Submitted to: Nature
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/1/2005
Publication Date: 12/22/2005
Citation: Machida, M., Asai, K., Sano, M., Tanaka, T., Kumagai, T., Terai, G., Kusumoto, K., Arima, T., Akita, O., Kashiwagi, Y., Abe, K., Gomi, K., Horiuchi, H., Kitamoto, K., Kobayashi, T., Takeuchi, M., Denning, D.W., Galagan, J.E., Nierman, W.C., Yu, J., Archer, D., Bhatnagar, D., Cleveland, T.E., Gotoh, O., Horikawa, H., Hosoyama, A., Ichinomiya, M., Igarashi, R., Iwashita, K., Juvvadi, P., Kato, M., Kato, Y., Kin, T., Kokubun, A., Maeda, H., Maeyama, N., Maruyama, J., Nagasaki, H., Nakajima, T., Oda, K., Okada, K., Paulsen, I., Sakamoto, K., Sawano, T., Takahashi, M., Takase, K., Terabayashi, T., Wortman, J., Yamada, O., Yamagata, O., Anazawa, H., Hata, Y., Koide, Y., Komori, T., Koyama, Y., Minetoki, T., Suharnan, S., Tanaka, A., Isono, K., Kuhara, S., Ogasawara, N., Kikuchi, H. 2005. Genome sequencing and analysis of Aspergillus oryzae. Nature. 438(7071):1157-1161.

Interpretive Summary: Aflatoxins are the most toxic and cancer-inducing compounds produced by certain fungi such as Aspergillus flavus and A. parasiticus. These compounds contaminate our food source such as corn, cotton, peanut, and tree nuts when the fungus infects crops. Understanding of the genetic and biochemical mechanisms of aflatoxin formation will provide valuable information on the control or elimination of aflatoxin contamination of food and feed. Aspergillus oryzae is an important industrial fungus used in the production of oriental fermented foods. A. oryzae is a close relative of A. flavus, which produces aflatoxins. The genome sequence of A. oryzae reveals a wealth of genetic information that scientists and researchers can compare with A. flavus and use to identify genes potentially involved in aflatoxin formation in A. flavus.

Technical Abstract: The genome of Aspergillus oryzae, an important industrial fungus used in the production of oriental fermented foods, such as soy sauce, miso, and sake, has been sequenced. The genome sequence reveals a wealth of genes encoding secreted enzymes. A comparison with the genome sequences of A. nidulans and A. fumigatus reveals that the A. oryzae genome is significantly larger than the other two genomes (6 Mb). In total, about 14,000 genes have been predicted, which is also 40 percent more than the other two genomes. This could be the result of acquisition during thousands of years of demastication and cultivation. Genes encoded by this acquired sequence are enriched for those predicted to be involved in metabolism, particularly in the synthesis of secondary metabolites. There is specific expansion of genes for secretary hydrolytic enzymes, amino acids metabolisms, and amino acids and sugar uptake transporters supporting the contention that A. oryzae is one of the ideal microorganisms for industrial fermentation.