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Title: DEVELOPMENT OF SEQUENCE-CHARACTERIZED AMPLIFIED REGION (SCAR) MARKERS FOR SUCROSE SYNTHASE-2 IN A POPULATION OF SACCHARUM OFFICINARUM X S. SPONTANEUM

Author
item Veremis, John
item Lingle, Sarah

Submitted to: American Society of Plant Biologists Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/2005
Publication Date: 7/20/2005
Citation: Veremis, J.C., Lingle, S.E. 2005. Development of Sequence-Characterized Amplified Region (SCAR) Markers for Sucrose Synthase-2 in a Population of Saccharum Officinarum x S. Spontaneum [abstract]. The American Society of Plant Biologists:Plant Biology 2005. Abs#963:283. Also available: http://abstracts.aspb.org/pb2005/public/P71/7558.html

Interpretive Summary:

Technical Abstract: Sugarcane is an allopolyploid, an interspecific hybrid of Saccharum species complex. Few gene-specific markers are known in this complex. The Sus2 (sucrose synthase; homologous to maize Sh1) gene is more highly expressed in sucrose-storing sugarcane genotypes (S. officinarum) than low-sucrose wild genotypes (S. spontaneum). We identified polymorphic, 240 base pair insertion-deletions (indels), arbitrarily designated A through G, within the Sus2 promoter region of PIN 84-1 (S. spontaneum) and Muntok Java (Saccharum sp. hybrid). Sequences from the indels were used to develop PCR-based sequence-characterized amplified region (SCAR) markers for specific indels. The utility of five of the indel-specific primer pairs in identifying Sus2 alleles was tested in a population of 100 progeny of LA Stripe (S. officinarum; 2n=80) x SES 147b (S. spontaneum; 2n=64). We hypothesized that the SCAR marker for Indel A came from La Stripe, and was associated with sucrose concentration. This marker produced a 230 bp band in S. officinarum, including LA Stripe, but did not produce a product in SES 147b (S. spontaneum). Of the progeny, 76 had marker A230, while 24 did not, a segregation ratio of about 3:1. There was no correlation of this marker with Brix (soluble solids) in juice samples taken during the harvest season in three field replications of the population. The primer pairs for three other indels, E, F and G, produced numerous polymorphic bands each in LA Stripe and SES 147b, but none of them was correlated with juice Brix. The SCAR marker for indel B was not polymorphic in the LA Stripe x SES 147b population, but was polymorphic between Saccharum and Erianthus, a related grass of interest to sugarcane breeders in the development of sugarcane for biofuel production. This marker might be useful in identifying intergeneric hybrids as Erianthus germplasm is brought into sugarcane breeding programs.